TUBEX® TF Jawaban Pasti Diagnosa Tifoid

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Tubex

MUDAH & PRAKTIS

TUBEX®TF mengeksploitasi kemudahan dan kepraktisan seperti Widal maupun Aglutinasi Latex. [WHO]

KUALITAS DIAGNOSA TINGGI

TUBEX®TF mempunyai sensitifitas dan spesifisitas > 95% mendeteksi Salmonella typhi. [Lim P et.al.]

FLEKSIBEL

TUBEX®TF dirancang sangat cocok baik untuk penggunaan bed-side maupun rutin laboratorium diagnosa demam tifoid. [Lim P,oraczc. G. et. al.]

RELIABLE

TUBEX®TF dengan antigen LPS 0-9 S. typhi sangat spesifik immunodominant dan robust. [WHO]

INOVATIF

Metode lnhibition Magnetic Binding lmmunoassay (lMBI) memungkinkan pengoperasian semudah Widal serta secanggih ELISA. [Lim P et. al.]

ANTIGEN EPITOPE SPESIFIK

Epitope Monoclonal lgM Deteksi Ab lgM S.typhi spesifik α-D-Tyvelose Ag LPS 0-9.

PROSEDUR PENGUJIAN

Penetesan Brown
Reagent 45 μl
Penetesan Specimen-control 45 μl Inkubasi 2 menit Penetasn Blue Reagent 90 μl Homogenisasi
2 menit
Separasi Magnetic
5 menit dan
pembacaan hasil

 

FKUI

BACKGROUNDS

Diagnosis of typhoid fever is still a health problem in Indonesia. Blood culture of S. typhi which was regarded as gold standard only positive in 40-70% of cases suspected as typhoid fever. PCR as another diagnostic tool can not be widely used because of its expensive and limited facilities. Widal test which is widely used because of cheap and easy to perform is influenced by many factors, so it has only 60-80% sensitivity and specificity. So we need another serologic test which can give rapid accurate results but also high sensitivity and specificity.

TUBEX®TF TEST COMPARED TO WIDAL TEST IN DIAGNOSTICS OF TYPHOID FEVER



Surya H*, Setiawan B*, Shatri H**, Sudoyo A***, Loho T****

* Tropic Infection Division,
** Division of Psycosomatic,
*** Division of Hematology - Medical Oncology, Department of Internal Medicine,
**** Department of Clinical Pathology Faculty of Medicine University of Indonesia.

TUBEX®TF is one of several new serologic test that has been studied in several countries and proved to have high sensitivity and specificity result but it is not yet studied in Indonesia.

OBJECTIVES

To study sensitivity and specificity TUBEX®TF test compared to Widal test in diagnosis of typhoid fever which are already confirmed by PCR and or blood culture.

METHODS

We collected inpatients with suspected typhoid fever (according to Nelwan's typhoid score >/=8) from Dr. Cipto Mangunkusumo Hospital, Persahabatan Hospital, and Tangerang Hospital. Patients were interviewed, physically examined, taken blood sample for Widal, S. typhi culture, PCR S. typhi and TUBEX®TF test. Data were processed with SPSS13 statistical programme and plotted in frequency table and cross table.

RESULT

From May - October 2006, we collected 54 samples that fulfilled the requirment criteria, but 2 samples were excluded because of icteric samples. The 52 samples consisted of 27 (52%) males and 25 (48%) females with the highest frequency of age is 20-30 years old (53.8%). Result of TUBEX®TF compared to Widal test diagnosing typhoid fever i.e: sensitivity 100% and 53.1%, specificity 90% and 65%, Positive Predictive Value (PPV) 94.11% and 70.8%, Negative Predictive Value (NPV) 100% and 46.4%, Positive likelihood ratio 10 and 1.51, negative likelihood ratio 0 and 0.72. Area below ROC curve 0.950 for TUBEX®TF and 0.591 for Widal Test. Compared to Widal test, the TUBEX®TF test was found to be significant (p<0.05) using Chi square statistic test.

CONCLUSION

TUBEX®TF test is more sensitive and specific than Widal test in diagnosing typhoid fever. 

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